Detection of Bacterial Rhamnolipid Toxin by Redox Liposome Single Impact Electrochemistry

The detection of Rhamnolipid virulence factor produced by Pseudomonas aeruginosa involved in nosocomial infections is reported using the redox liposome single impact electrochemistry. Redox liposomes based on 1,2-dimyristoyl-sn-glycero-3-phosphocholine as a pure phospholipid and potassium ferrocyanide an encapsulated content are designed for interaction target toxin with lipid membrane sensing strategy. electrochemical principle weakening upon which leads at ultramicroelectrode to breakdown release/electrolysis its probe. We present proof concept sensitive fast submicromolar concentration detected after less than 30 minutes incubation liposomes, appearing current spikes chronoamperometry measurement.